Genetic polymorphisms Of EGFR and CYP19 genes among Iraqi breast cancer patients

Running title EGFR and CYP19 genes in breast cancer

Abstract

Objective: The study looked at the relationship between breast cancer and CYP19A1 and EGFR single nucleotide polymorphisms (SNPs).
Methods: The sequences of the CYP19A1 and EGFR genes were examined in ten breast cancer patient samples, corresponding to two genetic loci measuring 600 bp and 658 bp, respectively. We used a direct Sanger sequencing method on the amplified locus to look at the pattern of SNP variations we saw in the population we studied.

Results: The sequencing experiments revealed one variation in the intron-3 region, specifically 175T>A, identified as rs7175531, and two variations in the exon-13 region, namely 84A>G and 324G>A, designated as rs11536635 and rs2227983, respectively. The statistical analyses indicated that the identified polymorphism in both rs11536635 and rs2227983 SNPs demonstrated a significant correlation with the advancement of breast cancer.      
Conclusion: rs7175531 SNP in the CYP19A1 gene and the rs11536635 and rs2227983 SNPs in the EGFR gene exhibited a notable association as potential markers linked to the development of breast cancer in the examined Iraqi samples. It showed a strong link between these SNPs and the progression of this disease.